FIG. 2.

Inhibition of cell-free fatty acid synthesis by triclosan. Bloodstream form (BSF) and procyclic form (PCF) membranes were preincubated with various concentrations of triclosan and assayed for fatty acid synthesis. (A) Lipids were extracted and analyzed by TLC. 14:0, [³H]myristate marker; O, origin; Mal-CoA, malonyl-CoA. (B) Densitometric quantitation of fatty acids from panel A. (C) Chain length analysis of the fatty acids shown in panel A. Fatty acid methyl esters were prepared and analyzed by reverse-phase TLC. The labels 10:0, 14:0, and 18:0 indicate the positions of [methyl-³H]decanoate, [methyl-³H]myristate, and [methyl-³H]stearate, respectively.