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. 2004 Aug;3(4):862–869. doi: 10.1128/EC.3.4.862-869.2004

FIG. 2.

FIG. 2.

Expression and localization of gMURF2-II gene. (A) Northern blot of 1 μg (lane 1), 2.5 μg (lane 2), 5 μg (lane 3), and 10 μg (lane 4) of mtRNA probed with an oligonucleotide complementary to gMURF2-II (lanes 1 to 3) or an oligonucleotide complementary to the minicircle gRNA gA6(14) (lane 4). The gMURF2-II probe detects both the ∼65-nt gRNA transcript and the ∼1.3-kb ND4 mRNA, while the gA6(14) probe hybridizes to a gRNA band only. (B) Restriction digestion and Southern blot analysis of T. brucei genomic DNA. Three micrograms of DNA was digested with HindIII (lanes 1), HindIII plus EcoRI (lanes 2), or EcoR1 (lanes 3), separated in a 1% agarose gel, transferred to a Nytran membrane, and probed with an end-labeled gMURF2-II oligonucleotide (left). The blot was then stripped and rehybridized with an end-labeled gA6(14) probe (right). (C) Restriction maps of T. brucei maxicircle and gA6(14) minicircle (H, HindIII; E, EcoR1).