FIG. 3.

(A) Nucleotide sequence of gMURF2-II locus including the mapped 5′ ends of gMURF2-II and ND4 transcripts as well as the minor-strand CR4 gene (22). The 5′ end of the gRNA was determined by primer extension analysis using the gMURF2-IIR primer, indicated by the arrow underneath the sequence. The 3′ end of the gRNA indicated was obtained by sequencing RT-PCR products. The start codon for ND4 is underlined. (B) Primer extension mapping of gMURF2-II and ND4 5′ ends. An end-labeled gMURF2-IIR primer was extended in the presence of 1 μg of mtRNA (lane 1) or gel-purified gRNA (lane 2). The nucleotide sequence was obtained via primer extension of the total mtRNA in the presence of ddA or ddT (lanes 3 and 4, respectively). Extension products were run in an 8% acrylamide-urea gel. The 5′ ends of the gRNA and mRNA are indicated by arrows. Two minor mRNA products are marked with asterisks.