FIG. 2.
Northern blot analysis of LAC1 and LAG1 transcription. Total RNAs were prepared from the indicated strains, electrophoresed through formaldehyde-agarose gels, and transferred to a nylon membrane. 18S RNA was visualized by staining to ensure equal loading, and LAC1 or LAG1 transcripts were detected by probing the blot with 32P-labeled DNA fragments from each coding region. (A) LAC1 mRNA levels are shown with relevant genetic backgrounds indicated. Plasmids carrying wild-type PDR1 (pPDR1), PDR3 (pPDR3), or hyperactive PDR3 (pPDR3-11) are listed along with the empty vector control (pRS315). (B) LAG1 mRNA levels are shown.