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Recombinant Rox1p binds to an element in the LAC1 promoter. DNase I protection analysis was used as described in the text. A purine-specific chemical cleavage reaction (AG) was used to locate the Rox1p binding site (shown to the left). DNase I digestion was carried out in the absence of added protein (no protein) or with increasing amounts of Rox1p (indicated at the top).
