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Lorentzian broadening for room temperature X-band (9 GHz) EPR spectra of STM25-nR1 peptide fully labeled with the MTSL side chain (R1) at positions n = 7, 11, and 19 as a function of cholesterol content in POPE:POPC (1:2 molar ratio) LMVs. The samples of identical lipid and peptide composition but diamagnetically diluted with unlabeled STM25 to 25% of STM25-nR1 were used to obtain the reference unbroadened EPR spectra. See text for further details.
