TABLE 1.

Yeast strains

Strain	Genotype	Source or reference
JY426	MATa leu2-3,112 ura3-52 his4-34 fus1-Δ1 fus2-Δ3	Cold Spring Harbor Laboratory
JY429	MATα trp1Δ1 ura3-52 cyh2 fus1-Δ1 fus2-Δ3	Cold Spring Harbor Laboratory
PJ69-4A	MATa trp1-Δ901 leu2-3,112 ura3-52 his3-Δ200 gal4Δ gal80Δ GAL2-ADE2 LYS2::GAL1-HIS3 met2::GAL7-lacZ	25
SEY6210	MATα leu2-3,112 ura3-52 his3-Δ200 trp1-Δ901 lys2-801 suc2-Δ9	S. Emr
SEY6211	MATa leu2-3,112 ura3-52 his3-Δ200 trp1-Δ901 ade2 suc2-Δ9	S. Emr
RAY563	Same as SEY6210 with sph1-Δ1::HIS3	2
RAY567	Same as SEY6211 with sph1-Δ1::HIS3	2
RAY914	Same as RAY931 with pRS414CDC24 instead of pEG(KT)CDC24	37
RAY916	Same as RAY931 with pRS414cdc24-m1 instead of pEG(KT)CDC24	37
RAY918	Same as RAY928 with pRS414CDC24 instead of pEG(KT)CDC24	37
RAY928	Same as SEY6210 with cdc24-Δ1::loxP HIS5Spc loxP and pEG(KT)CDC24	37
RAY931	Same as SEY6211 with cdc24-Δ1::loxP HIS5Spc loxP and pEG(KT)CDC24	37
RAY950	MATα leu2-3,112 ura3-52 his3-Δ200 trp1-Δ901 lys2-801 ade2 cdc24::LEU2 with pRS416GalHis6CDC24	37
RAY1042	Same as RAY950 with pRS414CDC24 instead of pRS416GalHis6CDC24	37
RAY1044	Same as RAY950 with pRS414cdc24-m1 instead of pRS416GalHis6CDC24	37
RAY1046	Same as RAY950 with pRS414cdc24-m2 instead of pRS416GalHis6CDC24	37
RAY1052	Same as RAY931 but cdc24-Δ1::loxP	36
RAY1474	Same as RAY1052 with bud1Δ::loxP HIS5SpcloxP	36
RAY1487	Same as SEY6211 but cdc24::TRP1 CDC24 and URA3::GFPBUD1	36
RAY1657	Same as RAY950 with pRS414cdc24-1 instead of pRS416GalHis6CDC24	34
RAY1660	Same as RAY950 with pRS414cdc24-111-1 instead of pRS416GalHis6CDC24	34
RAY1681a	Same as RAY950 with pRS414cdc24-m5 instead of pRS416GalHis6CDC24	This study
RAY1683a	Same as RAY950 with pRS414cdc24-m6 instead of pRS416GalHis6CDC24	This study
RAY1685b	Same as RAY950 with pRS414cdc24[R416G] instead of pRS416GalHis6CDC24	This study
RAY1699	Same as RAY950 with pRS414cdc24[E423K/N446I] instead of pRS416GalHis6CDC24	This study
RAY1705b	Same as RAY950 with pRS414cdc24[E423K] instead of pRS416GalHis6CDC24	This study
RAY1716	Same as RAY950 with pRS414cdc24[R416G/N551K] instead of pRS416GalHis6CDC24	This study
RAY1718	Same as RAY950 with pRS414cdc24[R416G/E423K] instead of pRS416GalHis6CDC24	This study
RAY1724	Same as RAY950 with pRS414cdc24[R416G/E423K/N446I/N551K] instead of pRS416GalHis6CDC24	This study
RAY1740b	Same as RAY928 with pRS414cdc24[R416G] instead of pEG(KT)CDC24	This study
RAY1747b	Same as RAY931 with pRS414cdc24[R416G] instead of pEG(KT)CDC24	This study

^aOriginal isolated mutants.

^bThe cdc24-m6 and cdc24-m5 mutants were recreated by the R416G and E423K mutations, respectively, and were used for all analyses.

^cHIS5Sp, HIS5 from S. pombe.