Change in the phosphorylation state of proteins related to autophagy in eWAT from chronic alcohol-fed and pair-fed control mice. Quantitation of Western blots for ULK1, beclin, Atg7, Atg12/5, Atg3, p62 and LC3B-II and -I. Unless noted, values have been normalized to the total amount of the respective protein that did not change with alcohol (data not shown) or tubulin. Quantitation of Atg3 is not presented graphically as there was no difference between groups. Representative Western blots are presented (panels A and B) from eWAT isolated from two control and two alcohol-fed mice. Values are means ± SEM; n = 11 and 14, respectively. *P < 0.05 compared to time-matched control values.