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. 2016 Sep 2;7:1348. doi: 10.3389/fpls.2016.01348

FIGURE 6.

FIGURE 6

Transient overexpression of ScGluD2 in Nicotiana benthamiana leaves infiltrated with Agrobacterium EHA105 carrying 35S::00- (control) or 35S::ScGluD2 construct. (A) Conductivity measurement of 35S::ScGluD2-transiently expressing leaves at 24 and 48 h after agroinfiltration. (B) DAB (3,3′-diaminobenzidinesolution) staining of leaf tissues 48 h after infiltration. (C) Relative expression level of the ScGluD2 gene in 35S::ScGluD2-transiently expressing leaves at 24 h after agroinfiltration. (D) Relative expression level of the immunity associated marker genes in N. benthamiana after infiltration for 24 h, including the hypersensitive response (HR) marker genes NtHSR201, NtHSR203 and NtHSR515, the salicylic acid (SA) associated gene NtNPR1, the jasmonic acid (JA) associated genes NtPR-1a/c, NtPR2 and NtPR3, and the ethylene (ET) synthesis-dependent genes NtEFE26 and NtAccdeaminase. Expression level of NtEF1α is used as the internal control. All data are represented as mean of three replicates (n = 3) ± SE. Different lowercase letters indicated a significant difference (p-value < 0.05) compared to the control, as determined by the least-significant difference test.