Table 1.
Primer | Sequence (5′–3′) | Strategy |
---|---|---|
ScGluD2-cDNAF | GCACAAGGATATGTCGTC | RT-PCR |
ScGluD2-cDNAR | ATGCTTTACATCACTACAAATAGAA | RT-PCR |
ScGluD2-QF | TATTGCTGTGGGTAATGAGGTCC | qRT-PCR |
ScGluD2-QR | TTGAAAGTGGCAGCAGAGGGAG | qRT-PCR |
GAPDH-QF | CACGGCCACTGGAAGCA | qRT-PCR |
GAPDH-QR | TCCTCAGGGTTCCTGATGCC | qRT-PCR |
ScGluD2-SublocF | TGCTCTAGAATGTCGTCCAAGAGACTACA | Subcellular localization vector construction |
ScGluD2-SublocR | GGACTAGTCATCACTACAAATAGAATGGGC | Subcellular localization vector construction |
ScGluD2-1301F | ATAAGAATGCGGCCGCATGTCGTCC | Overexpression vector construction |
ScGluD2-1301R | CGGGATCCTTACATCACTACAAATAGAATG | Overexpression vector construction |
NtHSR201F | CAGCAGTCCTTTGGCGTTGTC | qRT-PCR |
NtHSR201R | GCTCAGTTTAGCCGCAGTTGTG | qRT-PCR |
NtHSR203F | TGGCTCAACGATTACGCA | qRT-PCR |
NtHSR203R | GCACGAAACCTGGATGG | qRT-PCR |
NtHSR515F | TTGGGCAGAATAGATGGGTA | qRT-PCR |
NtHSR515R | TTTGGTGAAAGTCTTGGCTC | qRT-PCR |
NtNPR1F | GGCGAGGAGTCCGTTCTTTAA | qRT-PCR |
NtNPR1R | TCAACCAGGAATGCCACAGC | qRT-PCR |
NtPR-1a/cF | AACCTTTGACCTGGGACGAC | qRT-PCR |
NtPR-1a/cR | GCACATCCAACACGAACCGA | qRT-PCR |
NtPR2F | TGATGCCCTTTTGGATTCTATG | qRT-PCR |
NtPR2R | AGTTCCTGCCCCGCTTT | qRT-PCR |
NtPR3F | CAGGAGGGTATTGCTTTGTTAGG | qRT-PCR |
NtPR3R | CGTGGGAAGATGGCTTGTTGTC | qRT-PCR |
NtEFE26F | CGGACGCTGGTGGCATAAT | qRT-PCR |
NtEFE26R | CAACAAGAGCTGGTGCTGGATA | qRT-PCR |
NtAccdeaminaseF | TCTGAGGTTACTGATTTGGATTGG | qRT-PCR |
NtAccdeaminaseR | TGGACATGGTGGATAGTTGCT | qRT-PCR |
NtEF1αF | TGCTGCTGTAACAAGATGGATGC | qRT-PCR |
NtEF1αR | GAGATGGGGACAAAGGGGATT | qRT-PCR |