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. 2016 Apr 1;7(5):680–688. doi: 10.1111/jdi.12505

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© 2016 The Authors. Journal of Diabetes Investigation published by Asian Association for the Study of Diabetes (AASD) and John Wiley & Sons Australia, Ltd

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Effect of vitamin D receptor (VDR) small interfering ribonucleic acid (siRNA) transfection on podocytes. After transfecting with VDR‐siRNA of target 1 or target 2 at the concentrations of 20 nmol/L transfection solution, decreases were observed both in (a) VDR messenger RNA (mRNA) and (b) protein expression compared with those of mock or negative siRNA transfection, respectively (P < 0.05). The target 2 siRNA was chosen as the experimental VDR‐siRNA because of a more thorough inhibition of VDR. After VDR‐siRNA transfection, transforming growth factor‐β (TGF‐β) and angiotensinogen (AGT) expression were further elevated at both (c) mRNA and (d) protein levels (P < 0.05), whereas the vascular endothelial growth factor (VEGF) expression levels did not change significantly (P > 0.05). NCsi, negative control‐siRNA; VDRsi, VDR‐siRNA.