Fig. 1.

Human iPSC induction towards mesoderm by the newly identified GSK3β inhibitor CP21R7. (A) Outline of the three-step differentiation protocol to obtain hiPSC-derived podocytes; (B) CP21R7 chemical structure; (C) immunofluorescence analysis of BRY and OCT4 displaying commitment to mesodermal fate and reduction of pluripotency. Scale bar 50 μm; (D) gene expression analysis confirmed the induction towards mesoderm through an increase in T (BRY) expression and the loss of pluripotency through a decrease in NANOG expression. Data are expressed as mean ± s.e.m.