Effect of FGFR1 exon 9 and exon 8 knockdown on FGF23-regulated phosphate transport.
A, FGFR1 knockdown by siRNA for exon 8 (si8), exon 9 (si9), or a scrambled control (scr) was assessed by immunoblotting using antibody that specifically recognizes FGFR1c. A representative result is presented. B, quantification of si8 and si9 knockdown of FGFR1c. Data represent the mean ± S.E. (error bars) of n = 4 experiments. C, siRNA effects on FGF23-sensitive phosphate transport. Data represent the mean ± S.E. of n = 4 experiments. *, p < 0.05; **, p < 0.01.