FIGURE 8.

shRNA NHERF1 knockdown inhibits FGF23- and PTH-regulated phosphate transport. NHERF1 expression was knocked down in RPTECs by shRNA transfection as described under “Experimental Procedures.” A, knockdown of NHERF1 was assessed by immunoblotting. A representative experiment is depicted. The data for NHERF1 shRNA and a scrambled control (scr) are shown. B, effects of NHERF1 knockdown on PTH- and FGF23-dependent phosphate transport were assessed. Data represent the mean ± S.E. (error bars) of n = 4 experiments. Data were normalized for each experiment, where phosphate uptake under control, untreated conditions was defined as 0% inhibition. **, p < 0.01 versus FGF23 or PTH.