TABLE 2.
SAXS data collection and derived parameters
| Plectin SR3–SR6 | Plectin SR7–SR9 | Plectin SR3–SR9 | Desmoplakin SR7–SR9 | Desmoplakin SR3–SR9 | |
|---|---|---|---|---|---|
| Data collection | |||||
| Concentration range (mg ml−1) | 1.7–26.5 | 1.5–12.3 | 0.92–14.7 | 0.96–7.7 | 0.59–4.7 |
| Exposure time (s) | 20 × 0.05 | 20 × 0.05 | 20 × 0.05 | 30 × 0.05 | 30 × 0.05 |
| Structural parameters | |||||
| Guinier region qRg range | 0.21–1.20 | 0.22–1.00 | 0.46–1.07 | 0.39–1.30 | 0.45–1.30 |
| I(0)/c (10−2 cm2 mg−1) (from Guinier)a | 3.03 ± 0.01 | 2.53 ± 0.01 | 5.85 ± 0.04 | 2.91 ± 0.01 | 7.33 ± 0.01 |
| Rg (Å) (from Guinier) | 50.4 ± 0.6 | 42.3 ± 0.3 | 85.4 ± 2.0 | 43.7 ± 0.3 | 72.7 ± 1.0 |
| Rc (Å) (from modified Guinier) | 9.34 ± 0.01 | 9.23 ± 0.02 | 9.37 ± 0.01 | 9.33 ± 0.01 | 9.80 ± 0.02 |
| I(0) (10−2 cm2 mg−1)a (from P(r)) | 3.11 ± 0.01 | 2.55 ± 0.01 | 5.93 ± 0.02 | 2.96 ± 0.01 | 7.60 ± 0.01 |
| Rg (Å) (from P(r)) | 53.9 | 44.1 | 90.8 | 47.2 | 83.4 |
| Dmax (Å) (from P(r)) | 210 | 170 | 350 | 175 | 350 |
| Rc (Å) (from Pc(r)) | 9.4 | 10.0 | 10.3 | 10.5 | 11.4 |
| Dc (Å) (from Pc(r)) | 35 | 44 | 45 | 50 | 50 |
| Porod volume, Vp (Å3) | 90,624 | 57,400 | 135,300 | 69,400 | 136,000 |
| Excluded volume, Vex (Å3) | 98,350 ± 670 | 77,810 ± 460 | 187,500 ± 830 | 89,300 ± 470 | 234,100 ± 2,900 |
| Molecular mass (kDa) (from (Vp/1.6)) | 56.6 | 35.9 | 84.6 | 43.4 | 85.0 |
| Molecular mass (kDa) (from (Vp/2)) | 49.2 ± 0.3 | 38.9 ± 0.2 | 93.7 ± 0.4 | 44.7 ± 0.2 | 117.1 ± 1.5 |
| Monomeric mass from sequence (kDa) | 53.2 | 42.8 | 95.6 | 46.8 | 99.9 |
| SASBDB codeb | SASDBA4 | SASDBB4 | SASDBC4 | SASDBD4 | SASDBE4 |
a Absolute intensities were determined using water as a secondary standard.
b SASBDB, Small Angle Scattering Biological Data Bank.