FIGURE 7.

CAR KO mice do not have accumulation of ER proteins after withdrawal of TCPOBOP administration. Male CAR KO and WT mice were treated with TCPOBOP (3 mg/kg) once a day for 3 consecutive days, and mice were sacrificed 3 days after withdrawal of TCPOBOP administration. A and B, the ratio of the liver to body weight (A) and serum ALT levels (B) were quantified. Data are presented as mean ± S.E. (n = 3). ns, no statistical difference (Student's t test). C and D, total liver lysates were subjected to Western blotting analysis (C) followed by densitometry analysis (D). All proteins were normalized to the loading control β-actin are and presented as -fold over vehicle control groups (mean ± S.E., n = 3). *, p < 0.05; one-way ANOVA. E, liver samples were processed for EM, and representative images are shown. Arrows, ER; N, nucleus; M, mitochondrion. F, ER areas in the cytosol were quantified using ImageJ software as described under “Materials and Methods.” Data are presented as the percentage of the volume of the ER versus cytosol area (mean ± S.E., n = 3). EM images from three different mice are shown, and more than 10 cell sections were randomly selected and quantified in a blinded fashion from each mouse. *, p < 0.05 (Student's t test).