FIGURE 3.

α-Syn fibrils cause seed-dependent aggregation in vivo. Distribution of phosphorylated α-syn pathology in mouse brain. WT mouse brain was injected with α-syn samples (10 μg) into striatum and observed 3 months later (n = 7 for 37 °C shaking, n = 5 for 37 °C standing, n = 3 for room temperature (RT) standing, n = 2 for 4 °C standing). Phosphorylated α-syn pathology was evaluated by immunohistochemistry with Ser(P)-129 antibody 1175. Typical images of brain injected with 37 °C shake (left panel) and 37 °C stand (right panel) are shown (top panels). Schematics of phosphorylated α-syn pathology at the level of 0.62 mm from the bregma are shown (bottom panels). Red dots indicate phosphorylated α-syn pathology. Scale bar = 25 μm.