FIGURE 7.

In vivo seeding activity of α-syn fragmented by sonication. A, distribution of phosphorylated α-syn pathology in brains of mice observed 3 months after injection of α-syn samples (10 μg) into striatum (Str). Phosphorylated α-syn pathology was evaluated by immunohistochemistry with Ser(P)-129 antibody. Typical images (right panels) and schematics of phosphorylated α-syn pathology (left panels next to images) at the level of 0.62 mm from the bregma of brain injected with α-syn fibrils sonicated for 0 s (n = 7, top left panel), 15 s (n = 3, top right panel), 60 s (n = 3, bottom left panel), and 180 s (n = 3, bottom right panel) are shown. Red dots indicate phosphorylated α-syn pathology. Scale bar = 100 μm. B, quantification of α-syn pathology in mice injected with α-syn fibrils into striatum. The results are expressed as mean ± S.E. (n = 3). *, p < 0.05; **, p < 0.01; Student's t test against the value of 0 s. C, ThT staining in striatum of mice injected with α-syn fibrils sonicated for 180 s into striatum. Scale bar = 25 μm. D, widespread phosphorylated α-syn pathology in mice injected with α-syn fibrils sonicated for 180 s into striatum. Scale bar = 20 μm.