FIGURE 2.
Genetic deletion and complementation of the slr1796 gene of Synechocystis 6803. A, scheme of slr1796 replacement by a KmR cassette. B, PCR confirmation of complete segregation of the Δslr1796 mutant. Primer positions are indicated. Primer pair p3 + p4 is specific to the slr1796 gene (0.37-kb product) but not to KmR. The expected size of the PCR products from primers p1 + p2 is 2.0 kb in WT and 2.6 kb in the mutant. M, DNA size standard. C, construction of the Comp strain. Slr1796 was fused at the C terminus with a His6 tag and expressed under the Pcpc560 promoter in the Δslr1796 background.