FIGURE 3.
Phenotypic characterization of WT, the Δslr1796 mutant, and the complemented strain of Synechocystis 6803. A, growth curves of three strains grown under 40 μmol of photons m−2·s−1. Error bars represent the standard deviation of three biological replicates. B, strains grown in liquid culture to the same OD730 nm showing differences in the culture color. C, whole cell absorption spectra of three strains adjusted to equal cell numbers. Absorption peaks for chlorophyll a are at ∼440 and 680 nm and for phycobilins at ∼620 nm. D, low temperature (77 K) chlorophyll fluorescence emission spectra. The excitation wavelength was 435 nm. Peaks at 685 and 695 nm arise from PSII; the peak at 721 nm arises from PSI. For C and D, three or more independent replicates were performed, and representative data are presented. r.u., relative units.