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. 2016 Jul 5;291(36):18689–18699. doi: 10.1074/jbc.M116.721175

FIGURE 5.

FIGURE 5.

Transcript levels of PSI and PSII genes in WT and Δslr1796 mutant cells. The constitutively expressed rnpB gene was used as a reference and to show that samples do not contain contamination from genomic DNA when no reverse transcriptase (RTase) was added. The primer pair used for the detection of slr1796 gene was p3 + p4 as in Fig. 2A. Optimal PCR cycles were determined for each primer pair to ensure that the amplifications were in the linear range. Three or more independent replicates were performed, and representative results are shown.