Subcellular localization of the Slr1796 protein. Protein fractions were prepared from the Comp strain. A, immunoblotting analysis of the cellular localization of the Slr1796-His protein using His tag antibodies. Lane 1, total cellular protein; lanes 2 and 3, pellet and soluble fractions after centrifugation; lanes 4 and 5, plasma and thylakoid membranes isolated by two-phase partitioning, respectively, that were validated by the detection of specific marker proteins NrtA and CP47, respectively. B, immunoblotting detection of Slr1796-His in pellet (P) and soluble (S) fractions of thylakoid membrane treated with the indicated solubilizing agents. D1 was used as a control integral membrane protein, and PsaE was used as a control extrinsic protein.