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. 2016 Jul 5;291(36):18689–18699. doi: 10.1074/jbc.M116.721175

TABLE 2.

Oligonucleotide primers used in this study

Product Forward (5′–3′) Reverse (5′–3′)
slr1796 left flank cagctatgaccatgattacgaattcccaaacagccccccagg ttcttcacgacgtggaaaaaactcagtctaaaataggg
Kanamycin gene tttttccacgtcgtgaagaaggtgttgctgactca aaagaatgagggaaagccacgttgtgtct
slr1796 right flank gtggctttccctcattctttccgacaacaaaaaaacgga atccccgggtaccgagctcgaattcggatggggacgctgaagccg
slr1796 deletion detection (p1 + p2) ccaagcttgttgatgcggcc cctccagtggaagtggcgagt
slr1796 detection (p3 + p4) atcgccgctttggaagatga atcgccgctttggaagatga
slr1796-His6 cataaagtcaagtaggagattaattcaatgattatgtctgtg catgagaccggtctcagtgatgatgatggtggtgatcgactaattcac
pcpc560-Slr179His6 vector backbone tgagaccggtctcatgataaccagg tgaattaatctcctacttgactttatgagttgg
psaA gctaaggccaaagtctcggt tgggcattggcatggagatt
psaB tgtactccctgccctcctac ggaagcccaagaagagggac
psbA cccccggtggatattgatgg accccagctacccctaacat
psbB tgcccaggaatggattggtc acaccggtttgttccacact
psaC gcagccaacacaatcctcgg ggttgtacccaatgcgtccg
PsaD accagcgcctctgaacaagt cgtagggggctttaccggag
psaE ggtgattgtgcgcttcgacc tattttgccgccgcttgcac
psaF gccaagggccaaaggaaacc ccccgaaggttatccccacc
psaI acggttcctacgctgcatct agcaaccccatggtcacagc
psaJ taggggtggaaaagaagatcggg cctttttgtcaactgctccggt
psaK1 gccctaaagttgctggggtg ccagcacagcccctaaacca
psaK2 aactggggcagagccaagtc gcgctcaaagagggggattg
psaL gggcatctttccacccccat gccatgcccacttccaaacc
psaM atggcattatccgacacccaaat cggaaagccaggaaagcagg
btpA gaccaccgctggatctaccc gagcgagcggaacaagaagc
ycf3 agaggcgttacggttggagg gcccggtgattttgagccag
ycf4 tggtcacttgcccggacttt agaagtattgggagcccgcc
ycf37 tgggctatgcctgtttcgct gaattggcccgccgttgag
rnpB agttagggagggagttgc taagccgggttctgttcc