TABLE 2.
Oligonucleotide primers used in this study
Product | Forward (5′–3′) | Reverse (5′–3′) |
---|---|---|
slr1796 left flank | cagctatgaccatgattacgaattcccaaacagccccccagg | ttcttcacgacgtggaaaaaactcagtctaaaataggg |
Kanamycin gene | tttttccacgtcgtgaagaaggtgttgctgactca | aaagaatgagggaaagccacgttgtgtct |
slr1796 right flank | gtggctttccctcattctttccgacaacaaaaaaacgga | atccccgggtaccgagctcgaattcggatggggacgctgaagccg |
slr1796 deletion detection (p1 + p2) | ccaagcttgttgatgcggcc | cctccagtggaagtggcgagt |
slr1796 detection (p3 + p4) | atcgccgctttggaagatga | atcgccgctttggaagatga |
slr1796-His6 | cataaagtcaagtaggagattaattcaatgattatgtctgtg | catgagaccggtctcagtgatgatgatggtggtgatcgactaattcac |
pcpc560-Slr179His6 vector backbone | tgagaccggtctcatgataaccagg | tgaattaatctcctacttgactttatgagttgg |
psaA | gctaaggccaaagtctcggt | tgggcattggcatggagatt |
psaB | tgtactccctgccctcctac | ggaagcccaagaagagggac |
psbA | cccccggtggatattgatgg | accccagctacccctaacat |
psbB | tgcccaggaatggattggtc | acaccggtttgttccacact |
psaC | gcagccaacacaatcctcgg | ggttgtacccaatgcgtccg |
PsaD | accagcgcctctgaacaagt | cgtagggggctttaccggag |
psaE | ggtgattgtgcgcttcgacc | tattttgccgccgcttgcac |
psaF | gccaagggccaaaggaaacc | ccccgaaggttatccccacc |
psaI | acggttcctacgctgcatct | agcaaccccatggtcacagc |
psaJ | taggggtggaaaagaagatcggg | cctttttgtcaactgctccggt |
psaK1 | gccctaaagttgctggggtg | ccagcacagcccctaaacca |
psaK2 | aactggggcagagccaagtc | gcgctcaaagagggggattg |
psaL | gggcatctttccacccccat | gccatgcccacttccaaacc |
psaM | atggcattatccgacacccaaat | cggaaagccaggaaagcagg |
btpA | gaccaccgctggatctaccc | gagcgagcggaacaagaagc |
ycf3 | agaggcgttacggttggagg | gcccggtgattttgagccag |
ycf4 | tggtcacttgcccggacttt | agaagtattgggagcccgcc |
ycf37 | tgggctatgcctgtttcgct | gaattggcccgccgttgag |
rnpB | agttagggagggagttgc | taagccgggttctgttcc |