Cryo-TEM of WT C. glutamicum (A) and the conditional knockdown strain CGLcKD-0847 (B). CGLcKD-0847 was grown in the presence of 25 μm IPTG. Black arrowheads, black arrows, and white arrows, plasma membrane, cell wall, and outer membrane, respectively. White arrowheads, detached membrane fragments in the conditional knockdown. Scale bar, 300 nm.