TABLE 2.
MIC values of various antibiotics against the cpsA1 and cpsA2 knock-out mutants of M. tuberculosis H37Rv mc26206 and M. tuberculosis CDC1551
MICs were determined in 7H9-ADC-tyloxapol (M. tuberculosis H37Rv mc26206) or 7H9-OADC-Tween 80 (M. tuberculosis CDC1551) broth. MIC values are in μg/ml. INH, isoniazid; EMB, ethambutol; CIP, ciprofloxacin; VAN, vancomycin; RIF, rifampicin; STR, streptomycin; AMP, ampicillin; PEN G, penicillin G; CLAV, clavulanate (used at 5 μg/ml). ND, not determined; NA, not applicable due to the fact that a streptomycin resistance cassette was used in the construction of the mutant strain. MIC determinations were performed at least twice on independent culture batches.
| Strains | MIC |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| INH | EMB | CIP | VAN | RIF | STR | CPZEN-45 | PEN G | PEN G/CLAV | AMP | AMP/CLAV | |
| μg/ml | |||||||||||
| M. tuberculosis H37Rv mc26206 | 0.02 | 4 | 0.8 | 25 | 0.1 | 8 | 0.2–0.4 | 600 | 37.5 | 150–300 | 37.5 |
| mc26206ΔRv3267 | 0.04 | 2 | 0.4 | 0.78 | 0.003 | NA | 0.025 | 300 | 9.4 | 75–150 | 9.4 |
| mc26206ΔRv3267-compl | 0.04 | 4 | 0.8 | 25 | 0.1 | NA | 0.4 | 600 | 37.5 | 300 | 37.5 |
| mc26206ΔRv3484 | 0.02 | 2 | 1.6 | 25 | 0.1 | 4 | 0.2 | 600 | 18.7 | 300 | 18.7 |
| mc26206ΔRv3484-compl | 0.02 | 4 | 0.8 | 25 | 0.1 | 8 | 0.2 | 600 | 37.5 | 300 | 9.4 |
| M. tuberculosis CDC1551 | ND | 2 | 0.4 | 3.12 | 0.012 | 4 | 0.4–0.8 | 600 | 9.4–18.8 | 150 | 18.8 |
| CDC1551ΔRv3267 | ND | 4 | 0.2 | 1.6 | 0.0063 | NA | 0.1 | 37.5 | 4.7 | 75 | 9.4 |
| CDC1551ΔRv3267-compl | ND | ND | ND | ND | 0.025 | NA | 0.4–0.8 | 600 | 37.5 | 150–300 | 37.5 |
| CDC1551(Rv3267::Tn) | ND | 1 | 0.2 | 1.6 | 0.0031 | 8 | 0.1 | ND | ND | ND | ND |
| CDC1551(Rv3267::Tn)-compl | ND | 1 | 0.4 | 6.25 | 0.05 | 4 | 0.4 | ND | ND | ND | ND |
| CDC1551ΔRv3484 | ND | 2 | 0.4 | 6.25 | 0.012 | 4 | 0.4–0.8 | ND | ND | ND | ND |
| CDC1551ΔRv3484-compl | ND | 2 | 0.4 | 6.25 | 0.012 | ND | 0.4 | ND | ND | ND | ND |