TABLE 1.
Dissociation constants (KD) obtained from BLI and/or MST measurements
KD were determined for UvrB, preincubated UvrBC, UvrC, and UvrBΔ4 complexes binding to 48-bp fragments of homoduplex DNA (dsDNA, white background), and DNA containing an 8-nt unpaired (bubble, dark gray background) region. The interaction between UvrB and UvrC (in the absence of DNA, light grey background) was probed for UvrC both (from B. caldotenax) and from T. maritima by fluorescently labeling UvrB (from B.caldotenax) in MST experiments. Representative data are shown in Supplemental Fig. S3. The errors signify the standard deviations from n contributing experiments. ND means not determined.
