TABLE 1.

Strains and plasmids used in this study

Strain	Relevant genotype	Source or Ref.
E. coli DH5α	recA1;endA1;gyrA96; thi;relA1;hsdR17 (rK−;mK+); supE44; f80ΔlacZΔM15;DlacZYA-argF; UE169	Novagen
E. coli BL21(DE3)	fhuA2[lon]ompT gal (λDE3)[dcm]ΔhsdSλDE3	Novagen
M. smegmatis mc2155	Wild-type (WT) M. smegmatis strain	52
MtbH37Rv	Wild-type (WT) M. tuberculosis strain	ATCC 25618
ΔphoPMtb H37Rv	ΔphoP::attP-kan-attP	Smith and co-workers (27)
Complemented ΔphoPMtb H37Rv	ΔphoP::pSM607	Smith and co-workers (27)
Plasmids
pET-15ba	E. coli cloning vector	Novagen
pET-phoP	His6-tagged PhoP expression plasmid	58
pET-espR	His6-tagged EspR expression plasmid	This study
pET-28cb	E. coli cloning vector	Novagen
pET-crp	His6-tagged CRP expression plasmid	This study
p19Kproc	Mycobacteria expression vector	56
p19Kpro-espACD	Entire 1–3233 bp espACD operon cloned in p19Kpro	This study
p19Kpro-espB	EspB residues 1–460 cloned in p19Kpro	This study
p19Kpro-espR	EspR residues 1–132 cloned in p19Kpro	This study
p19Kpro-phoP	PhoP residues 1–247 cloned in p19Kpro	This study
pME1mL0031b	Mycobacterial protein expression vector	61
pME1mL1-phoP	PhoP residues 1–247 cloned in pME1mL1	This work
pSM128d	Integrative promoter probe vector for mycobacteria	57
pSM-espAup	espAup-lacZ fusion in pSM128	This work
pSM-espAupmut	Mutant espAup-lacZ fusion carrying changes in the PhoP-binding site	This work
pRSF-Duet-1b	E. coli cloning vector	Novagen
pRSF-Duet-phoP-espR	S-tagged PhoP and His-tagged EspR expression plasmid	This study
pUAB400 b	Integrative mycobacteria, E. coli shuttle plasmid	48
pUAB400-phoP	PhoP residues 1–247 cloned in pUAB400	55
pUAB300c	Episomal mycobacteria, E. coli shuttle plasmid	48
pUAB300-espR	EspR residues 1–132 cloned in pUAB300	This study
pUAB300-phoR	PhoR residues 1–485 cloned in pUAB300	55

^a Amp^r is ampicillin resistance.

^b Kan^r is kanamycin resistance.

^c Hyg^r is hygromycin resistance.