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. 2016 Jul 11;291(36):19092–19107. doi: 10.1074/jbc.M116.722132

FIGURE 6.

FIGURE 6.

PTX inhibits the Wnt-5a-induced activation of the non-canonical Wnt pathway in hippocampal neurons. A, representative Western blotting and quantification of total and phosphorylated levels of CaMKII (n = 4), JNK (n = 4), and PKCβII (n = 6) and the total levels of β-catenin (n = 3) and PSD-95 (n = 4) in neurons preincubated with PTX for 6 h and then treated with Wnt-5a for the indicated lengths of time. β-Actin and β-tubulin were used as loading controls. B, representative images of neurites of hippocampal neurons at DIV 14 treated with recombinant Wnt-5a ligand, with PTX or with PTX plus Wnt-5a or untreated for 2 h. PSD-95 is shown in green, and fluorescent phalloidin-labeled actin is shown in blue. Quantification of the number of PSD-95 clusters/10 μm/neurite after Wnt-5a and/or PTX treatment for the indicated lengths of time (n = 3, 10 neurons/condition, 3 neurites/neuron) is shown. **, p < 0.01; *, p < 0.05.