FIGURE 8.

Gallein blocks the Wnt-5a-induced changes in dendritic spine density. A, measurement of the increases in intracellular Ca²⁺ in the hippocampal neurons that were bathed in a Ca²⁺-free solution and treated with Gallein or Wnt-5a plus Gallein (n = 3). B, quantification of area under the curve of the measurement of Ca²⁺ concentrations. C, images of neurons treated with Wnt-5a and/or Gallein for 2 h. Quantification of the dendritic spine density. D, the neurons were transfected with EGFP or EGFP + βARKct and treated with Wnt-5a for 2 h. Quantification of the dendritic spine density (n = 3) is shown. Scale bar, 3 μm. **, p < 0.001; **, p < 0.01. ns, no significant difference.