FIGURE 4.

PSAK and LHCSR3 accumulation is dependent on CGL78. A, the abundance of the α and β subunits of CF₁, appearing as a single band, were visualized as a loading control (bottom panel). Membrane fractions, equivalent to 10 μg of protein, were loaded on sodium dodecyl sulfate-containing denaturing gels. B, samples used for panel A were re-evaluated for estimating relative abundances. An asterisk marks the copper-replete sample, whereas the circle represents the copper-depleted sample from either cgl78-ami11 cells that were grown in medium containing nitrate as nitrogen source. C, shown are the relative mRNA abundances of CRD1, CTH1 (2.1 kb functional transcript), CTH1 (3.1 kb non-functional transcript) in either CGL78 (black squares) or cgl78-ami11 (white apex-down triangle or cgl78-ami13 (white apex-up triangle) that were grown in copper-depleted or copper-replete medium as indicated. D, CGL78 mRNA abundance in crd1 (white circles) or CRD1 (gray circles) was determined by qRT-PCR. Each symbol represents the average of a duplicate qPCR of an independent experiment.