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pH dependence of the signal intensities, ±5% estimated error, for the CD3 resonances of Ala7 (red circles, |Δνq| = 8 kHz) and Ala9 (black circles, |Δνq| = 28 kHz) of GWALP23-H12 in DOPC bilayers, at β = 90° sample orientation. The midpoints, shown by the dashed lines, correspond to 50% maximal peak intensity, observed at pH 2.3 for Ala9 or pH 2.6 for Ala7. Error bars indicate means ± 5% estimated error.
