Table 1. Differentially-expressed proteins in mitochondrial of liver in broiler chickens with (-)-HCA treatment identified by PMF query$.
| Spot No. | Gene Ontology | Protein name | Accession No. | Exp. MW(KDa)/pI | Matched peptides | Score | Expression change treated with (-)-HCA# |
||
|---|---|---|---|---|---|---|---|---|---|
| 1000 mg/Kg | 2000 mg/Kg | 3000 mg/Kg | |||||||
| 1 | NDUFA10 | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10 | gi|71895153 | 41.43/6.15 | 2 | 189 | ↑ | ↑ | / |
| 2 | DYPS | dihydropyrimidinase | gi|363731013 | 58.03/5.73 | 2 | 147 | ↑ | ↑ | / |
| 3 | NDUFS3 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 | gi|226437575 | 29.23/6.55 | 7 | 449 | ↑ | ↑ | ↑ |
| 4 | ACO2 | aconitate hydratase | gi|45383738 | 85.79/8.05 | 4 | 260 | ↑ | ↑ | |
| 5 | Prss1 | protease, serine, 1 precursor | gi|16716569 | 26.13/4.75 | 1 | 70 | ↑ | / | ↑ |
| 6 | N/A | Chain A, Crystal Structure Of The First Active Autolysate Form Of The Porcine Alpha Trypsin | gi|1942351 | 13.297.83 | 1 | 126 | ↓ | / | ↓ |
| 7 | HGD | homogentisate 1, 2-dioxygenase | gi|50729534 | 49.476.35 | 1 | 57 | ↓ | / | / |
| 8 | PC | pyruvate carboxylase | gi|45383466 | 127.25/6.26 | 7 | 479 | ↑ | ↑ | / |
| 9 | EETI-II | Chain E, Complex Of Eeti-II With Porcine Trypsin | gi|157878102 | 24.17/8.26 | 1 | 78 | ↑ | / | / |
| 10 | UQCRC2 | cytochrome b-c1 complex subunit 2, mitochondrial-like | gi|118098350 | 48.58/9.04 | 4 | 331 | ↑ | ↑ | ↑ |
| 11 | N/A | Chain B, Refined 1.8 Angstroms Resolution Crystal Structure Of Porcine Epsilon-Trypsin | gi|999627 | 8.82/6.67 | 1 | 137 | ↑ | ↑ | ↑ |
| 12 | AKR7A2 | aflatoxin B1 aldehyde reductase member | gi|118101125 | 36.62/6.76 | 2 | 110 | ↑ | / | / |
| 13 | PDHA1 | pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial precursor | gi|60302740 | 44.44/8.19 | 3 | 129 | ↑ | ↑ | / |
| 14 | ATP5H | ATP synthase subunit d, mitochondrial isoform 1 | gi|118099965 | 18.34/8.73 | 2 | 85 | ↑ | ↑ | / |
| 15 | MUC5AC | mucin-5AC, partial | gi|332267570 | 92.18/6.33 | 1 | 133 | ↑ | ↑ | ↑ |
| 16 | N/A | Chain A, Chicken Cytochrome Bc1 Complex Inhibited By An Iodinated Analogue Of The Polyketide Crocacin-D | gi|196049775 | 49.44/5.95 | 2 | 117 | ↑ | ↑ | ↑ |
| 17 | ALDH2 | aldehyde dehydrogenase, mitochondrial isoform 2 | gi|363739855 | 56.79/7.92 | 3 | 217 | ↓ | ↑ | / |
| 18 | PDIA3 | protein disulfide-isomerase A3 precursor | gi|45383890 | 56.18/5.76 | 2 | 281 | ↓ | / | ↓ |
| 19 | SUCLG2 | succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial | gi|57524960 | 46.76/6.49 | 4 | 282 | ↑ | ↑ | / |
| 21 | DLD | dihydrolipoyl dehydrogenase, mitochondrial | gi|71897021 | 53.98/8.19 | 2 | 188 | ↑ | / | / |
| 22 | DLST | dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial | gi|61098338 | 49.28/9.25 | 1 | 92 | / | ↑ | / |
| 23 | ECH1 | delta(3, 5)-Delta(2, 4)-dienoyl-CoA isomerase, mitochondrial-like, partial | gi|326936041 | 16.62/6.90 | 1 | 89 | / | ↓ | / |
| 24 | ALDH6A1 | methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial | gi|363734519 | 61.64/6.33 | 3 | 238 | / | ↓ | ↓ |
| 26 | NDUFS8 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial | gi|118090950 | 23.82/5.84 | 3 | 247 | / | ↓ | / |
| 27 | N/A | trypsinogen precursor | gi|242253868 | 25.88/6.85 | 1 | 127 | / | ↑ | ↑ |
| 28 | PRDX3 | thioredoxin-dependent peroxide reductase, mitochondrial | gi|363735594 | 25.76/8.58 | 2 | 190 | / | ↓ | / |
| 30 | PCK2 | phosphoenolpyruvate carboxykinase [GTP], mitochondrial precursor PEPCK | gi|45382653 | 70.97/8.16 | 5 | 240 | / | ↓ | / |
| 31 | KRT1 | unnamed protein product | gi|189054178 | 66.02/7.62 | 3 | 93 | / | ↑ | / |
| 32 | GLUL | glutamine synthetase | gi|45382781 | 42.14/6.38 | 1 | 60 | / | ↑ | / |
| 33 | LOC100655872 | delta(3, 5)-Delta(2, 4)-dienoyl-CoA isomerase, mitochondrial-like | gi|344298379 | 35.70/8.09 | 1 | 107 | / | ↑ | / |
| 34 | SDHA | succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial-like | gi|326917263 | 72.54/6.85 | 6 | 302 | / | ↓ | / |
| 36 | P4HB (PDI) | prolyl-4-hydroxylase (AA 5 - 494) | gi|63739 | 54.87/4.66 | 5 | 535 | / | ↑ | / |
| 37 | SLC25A13 | calcium-binding mitochondrial carrier protein Aralar2 | gi|61098440 | 74.10/8.93 | 2 | 246 | / | ↑ | ↑ |
| 38 | RGN | regucalcin | gi|45382019 | 33.23/5.77 | 1 | 160 | / | / | ↑ |
| 39 | SUOX | Chain A, Sulfite Oxidase From Chicken Liver | gi|3212610 | 50.94/5.53 | 4 | 329 | / | / | ↑ |
| 40 | TST | thiosulfate sulfurtransferase | gi|268370289 | 32.89/6.08 | 2 | 99 | / | / | ↑ |
| 41 | CAT | hypothetical protein RCJMB04_1j22 | gi|53127216 | 60.03/8.09 | 5 | 240 | / | / | ↑ |
| 42 | PDHB | pyruvate dehydrogenase E1 component subunit beta, mitochondrial | gi|310750374 | 38.88/5.95 | 3 | 164 | / | / | ↑ |
| 43 | CPOX | coproporphyrinogen-III oxidase, mitochondrial | gi|50729640 | 45.43/8.56 | 3 | 79 | / | / | ↑ |
| 44 | LOC395933 | sulfotransferase | gi|45384226 | 36.24/5.89 | 1 | 60 | / | / | ↓ |
$PMF: Peptide mass fingerprinting; pI: isoelectric point; MW: molecular weight.
#Compared with control group, ↑indicated up-regulated; ↓indicated down-regulated; /indicated no change.