Table 2. Differentially-expressed proteins in cytoplasm of liver in broiler chickens with (-)-HCA treatment identified by PMF query$.
| Spot No. | Gene Ontology | Protein name | Accession No. | Exp. MW(KDa)/pI | Matched peptides | Score | Expression change treated with (-)-HCA# |
||
|---|---|---|---|---|---|---|---|---|---|
| 1000 mg/Kg | 2000 mg/Kg | 3000 mg/Kg | |||||||
| 1 | PDXK | pyridoxal kinase | gi|363728772 | 35.01/5.93 | 3 | 347 | ↑ | ↑ | ↑ |
| 2 | PGK | phosphoglycerate kinase | gi|45384486 | 44.72/8.31 | 3 | 275 | ↑ | ↑ | ↑ |
| 3 | ARHGDIA | rho GDP-dissociation inhibitor 1 | gi|124249432 | 23.27/5.22 | 2 | 159 | ↑ | / | / |
| 4 | N/A | Chain A, Complex Of The Second Kunitz Domain Of Tissue Factor Pathway Inhibitor With Porcine Trypsin | gi|2914482 | 23.48/8.26 | 1 | 94 | ↑ | ↑ | / |
| 5 | ALB | ALB protein | gi|74267962 | 69.24/5.88 | 2 | 153 | ↑ | / | / |
| 6 | GART | trifunctional purine biosynthetic protein adenosine-3 | gi|47825387 | 106.54/7.51 | 2 | 111 | ↑ | ↑ | / |
| 7 | ADSL | adenylosuccinate lyase | gi|51874220 | 54.50/6.52 | 2 | 155 | ↑ | / | / |
| 8 | ANXA5 | Chain A, Crystal Structures Of Chicken Annexin V In Complex With Ca2+ | gi|62738641 | 36.07/5.61 | 5 | 469 | ↑ | / | ↑ |
| 9 | ALB | serum albumin precursor | gi|30794280 | 69.32/5.82 | 3 | 318 | ↑ | ↑ | ↑ |
| 10 | MAT1A | S-adenosylmethionine synthase isoform type-1 | gi|313760551 | 43.75/6.28 | 4 | 261 | ↑ | / | / |
| 11 | HBG2 | ORF | gi|212491 | 16.45/8.85 | 2 | 134 | ↓ | ↓ | / |
| 12 | ME1 | NADP-dependent malic enzyme | gi|45383538 | 62.00/6.45 | 1 | 66 | ↓ | / | ↓ |
| 13 | TPI1 | Chain A, Structure Of Triose Phosphate Isomerase From Chicken Muscle | gi|230359 | 26.54/7.26 | 2 | 130 | ↓ | / | / |
| 14 | PFAS | phosphoribosylformylglycinamidine synthase | gi|334323278 | 144.35/5.36 | 1 | 60 | ↑ | / | / |
| 16 | BHMT | betaine--homocysteine S-methyltransferase 1 | gi|50755288 | 45.01/7.56 | 4 | 523 | / | ↑ | / |
| 17 | PAH | phenylalanine-4-hydroxylase | gi|47604920 | 51.09/6.49 | 4 | 300 | / | ↑ | / |
| 18 | PCBD1 | pterin-4-alpha-carbinolamine dehydratase | gi|45382483 | 12.00/6.04 | 2 | 125 | / | ↑ | / |
| 19 | ALB | serum albumin precursor | gi|30794280 | 69.32/5.82 | 2 | 142 | ↑ | ↑ | ↑ |
| 20 | HMGCS2 | hydroxymethylglutaryl-CoA synthase | gi|363736325 | 55.17/8.51 | 3 | 192 | / | ↑ | / |
| 22 | CMBL | carboxymethylenebutenolidase homolog | gi|326917168 | 28.16/6.45 | 2 | 106 | / | ↓ | / |
| 23 | BPNT1 | 3′(2′), 5′-bisphosphate nucleotidase 1 | gi|308818127 | 32.91/5.60 | 1 | 143 | / | ↑ | / |
| 25 | GNMT | glycine N-methyltransferase-like | gi|327265472 | 33.29/6.10 | 2 | 152 | / | / | ↓ |
| 27 | CALR | calreticulin | gi|18389889 | 46.29/4.39 | 1 | 79 | / | / | ↑ |
| 28 | TST | thiosulfate sulfurtransferase | gi|268370289 | 32.89/6.80 | 2 | 192 | ↑ | ↑ | / |
| 29 | ECHS1 | enoyl-CoA hydratase precursor | gi|15982640 | 28.25/8.72 | 1 | 67 | ↑ | ↑ | ↑ |
| 30 | GAMT | guanidinoacetate N-methyltransferase | gi|363743812 | 23.74/5.56 | 3 | 200 | / | / | ↑ |
$PMF: Peptide mass fingerprinting; pI: isoelectric point; MW: molecular weight.
#Compared with control group, ↑ indicated up-regulated; ↓ indicated down-regulated; /indicated no change.