Table 1. SaCas9 and SpCas9-mediated mutant mice generation.
Target gene | Cas9 | Survived/Injected (%) | Survived embryos |
Pups (birth rate) | Mutant pups (%) | |
---|---|---|---|---|---|---|
Embryo Genotyped# | Transferred | |||||
Slx2 | SaCas9 | 122/166a (73.5%) | 24/27 | 95 | 17 (17.9%) | 16/17 (94.1%) |
SpCas9 | 204/280b (72.9%) | 14/26 | 178 | 17 (9.5%) | 8/17 (47.1%) | |
Zp1 | SaCas9 | 241/266a (90.6%) | 23/25 | 216 | 18 (8.3%) | 14/18 (77.7%) |
SpCas9 | 130/136b (95.6%) | 26/27 | 103 | 17* (16.5%) | 10/12 (83.3%) |
Note: All the pups were genotyped by Sanger sequencing.
#Mutant embryos/embryos tested.
*5 newborns were cannibalized by the mother mouse. a vs b, p > 0.05, Statistical significance was tested using χ2 test.