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. 2016 Sep 2;6:32565. doi: 10.1038/srep32565

Table 1. SaCas9 and SpCas9-mediated mutant mice generation.

Target gene Cas9 Survived/Injected (%) Survived embryos
Pups (birth rate) Mutant pups (%)
Embryo Genotyped# Transferred
Slx2 SaCas9 122/166a (73.5%) 24/27 95 17 (17.9%) 16/17 (94.1%)
SpCas9 204/280b (72.9%) 14/26 178 17 (9.5%) 8/17 (47.1%)
Zp1 SaCas9 241/266a (90.6%) 23/25 216 18 (8.3%) 14/18 (77.7%)
SpCas9 130/136b (95.6%) 26/27 103 17* (16.5%) 10/12 (83.3%)

Note: All the pups were genotyped by Sanger sequencing.

#Mutant embryos/embryos tested.

*5 newborns were cannibalized by the mother mouse. a vs b, p > 0.05, Statistical significance was tested using χ2 test.