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| Cas9 | gRNA | Total embryo No. | Triple-Targeted (%) | Double-Targeted (%) | Single-Targeted (%) | Non-Targeted (%) |
|---|---|---|---|---|---|---|
| SaCas9 | Tyr-G1 | 30 | 14 (46.7%) | 11 (36.7%) | 3 (10.0%) | 2 (6.7%) |
| Zp1 | ||||||
| Slx2 | ||||||
| SpCas9 | Tyr-G3 | 37 | 3 (8.1%) | 11 (29.7%) | 17 (50.0%) | 6 (16.2%) |
| Zp1 | ||||||
| Slx2 |
