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. 2016 Sep 2;6:32552. doi: 10.1038/srep32552

Figure 4. Determination of specific IgE and IgG mice serum antibodies at the end of the immunization protocol with rHev b 8.

Figure 4

(a) ELISA experiments for class determination for the two adjuvants used (Incomplete Freund’s and alum). The negative control corresponds to the mouse without sensibilization (*** = P < 0.001). (b) SDS-PAGE of the IgE (mAb 2F5) and a IgG mouse purified by an affinity column with rHev b 8.