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. 2016 Sep 2;6:32587. doi: 10.1038/srep32587

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Copyright © 2016, The Author(s)

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(a) Representative images of damaged lesions stained with Evans blue (upper) and hematoxylin/eosin (lower). (b) The lesion index was defined as the summed total area of damaged tissue. (c,d) Expression of IL-1β (c) and caspase-1 (d) was determined by real-time RT-PCR. The mRNA levels are expressed as ratios of the mean value for vehicle-treated wild-type mice. (e–i) Representative images of western blotting (e) and expression of pro-IL-1β (f) mature IL-1β (g) pro-caspase-1 (h) and cleaved caspase-1 (i). The protein levels were normalized to β-actin. n = 5–8; **P < 0.01; n. s., not significant; Veh, vehicle; Col, colchicine.