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. 2016 Sep 2;6:32644. doi: 10.1038/srep32644

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) The conceptual plasmid map of pET28a-P_tac-6pgdh. The DNA sequence of P_tac, lac operator, and RBS were shown as underlined, italic, and small, respectively. (b) SDS-PAGE analysis of 6PGDH expression in E. coli TOP10 and E. coli BL21(DE3). M, protein marker; Control, pET28a-P_tac; WT, pET28a-P_tac-6pgdh; P, purified 6PGDH. 6PGDH was indicated with an arrow.