Table 2. Kinetics parameters of 6PGDH and mutants.
| Mutations | Km [μM] | kcat [s−1] | kcat/Km [mM−1 *s−1] | Ratio kcat/Km | |||
|---|---|---|---|---|---|---|---|
| NADP+ | NAD+ | NADP+ | NAD+ | NADP+ | NAD+ | NAD+/NADP+ | |
| WT | 13. 9 ± 1.1 | 1397 ± 111 | 8.73 ± 0.02 | 12.6 ± 0.4 | 628.8 | 9.0 | 0.014 |
| R31T | 35.5 ± 1.7 | 605 ± 47 | 10.83 ± 0.13 | 9.12 ± 0.23 | 305.6 | 15 | 0.049 |
| R31I | 26.5 ± 1.3 | 354 ± 12 | 11.53 ± 0.15 | 15.01 ± 0.17 | 435.0 | 42.4 | 0.097 |
| R31I/T32G | 104.4 ± 5.4 | 362 ± 23 | 11.9 ± 0.16 | 12.94 ± 0.27 | 114.0 | 35.8 | 0.31 |
| A30C/R31I/T32K | 698 ± 49 | 404 ± 33 | 6.23 ± 0.18 | 6.0 ± 0.2 | 8.9 | 14.8 | 1.66 |
| A30E/R31I/T32D | 660 ± 64 | 127 ± 7 | 3.1 ± 0.1 | 10.8 ± 0.2 | 4.7 | 85.1 | 18.1 |
| A30D/R31I/T32I | 228 ± 16 | 11.87 ± 0.55 | 1.81 ± 0.05 | 5.75 ± 0.05 | 7.9 | 484.2 | 61.1 |
Each value represents the average of three independent measurements.