Figure 4. CK8 is a negative regulator of TLRs-induced NF-κB signaling.

(A–C) HT29 cells were transfected with increasing amounts of CK8 expression vector and treated with the indicated TLRs inducers (Pam3csk4, 10 μg/ml; Poly (I:C), 10 μg/ml, and CpG, 10 μg/ml) for 24 h. NF-κB activity was measured using luciferase reporter assay. *p < 0.05, **p < 0.01.