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. 2016 Sep 2;6:32710. doi: 10.1038/srep32710

Figure 6. TLR-induced NF-κB signaling activation was increased in CK8−/− mice.

Figure 6

(A) Colon tissues from CK8+/+ and CK8−/− mice were treated with LPS for 10min and the expression levels of p-IκBα, IκBα, and CK8 was analyzed using Western blot. GAPDH was used as internal control. (B) NF-κB activity was measured in colon tissues from CK8+/+ and CK8−/− mice. (C–E) The mRNA levels of TNFα, IFNγ and MCP in colon tissue from CK8+/+ or CK8−/− mice were measured using Realtime PCR. Results were normalized to the expression of GAPDH. *p < 0.05, **p < 0.01, ***p < 0.001.