Table 5.
Sample size calculation in a hypothetical experiment based on the coefficients of variation estimated in this study. Power was set at 0.80, α=0.05 and the minimal detectable difference to a fold-change of 1.5
| Parameter | Number of mice per group to be able to detect a difference >1.5-fold between two groups (e.g., treatment versus control) | |||
|---|---|---|---|---|
|
|
||||
| BALB/c (inbred) | C57BL/6 (inbred) | NMRI (outbred) | CD-1 (outbred) | |
| AOCglucose0-40 | 9 | 4 | 8 | 7 |
| Maximal blood glucose effect of HI | 4 | 3 | 6 | 5 |
| Body weight change | 7 | 8 | 23 | 19 |
| Fat mass | 40 | 50 | 47 | 52 |
| Lean mass | 3 | 3 | 3 | 3 |
| Liver weight | 3 | 4 | 4 | 4 |
| Epididymal fat mass | 8 | 22 | 34 | 32 |
Sample size calculations were performed in SAS JMP. HI=Human insulin. AOC=Area over the Δblood glucose vs. time curve.