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Primer sequences were as follows
| Gene | Primer sequences 5’ to 3’ | Length (bp) |
|---|---|---|
| RORγ-t | F-GAGCAATGGAAGTCGTCCTAGTCAG | 190 |
| R-AGGGCAATCTCATCCTCAGAAAA | ||
| Foxp-3 | F-TCACCTATGCCACCCTTATCC | 236 |
| R-GCTCCTCTTCTTGCGAAACTC | ||
| IFN-γ | F-GCTCTGAGACAATGAACGCTACA | 150 |
| R-TTTCTTCCACATCTATGCCACTT | ||
| IL-4 | F-TCCTGCTCTTCTTTCTCG | 101 |
| R-TTCTCCTGTGACCTCGTT | ||
| BATF | F-TGCCCTCATACCTCTACCCA | 151 |
| R-TCCAGTACATTGGCTCGAC | ||
| GAPDH | F-GACAAAATGGTGAAGGTCGGT | 120 |
| R-GAGGTCAATGAAGGGGTCG |
