Table 1. Summary of the information related to the different localities surveyed and genetic variability for the 16S for the three different Ophiothrix species.

Population	Code	Depth (m)	Coordinates	Geographical area	N	H	p	Hd	π	R2
Ophiothrix fragilis
Kristineberg†	KRI	Intertidal	58°14′27.32“N 11°25′55.14“E	NE Atlantic- North Sea	22	9	5	0.749 ± 0.094	0.00368 ± 0.0007	0.1467**
Roscoff	ROS	Intertidal	48°45′9.55“N 3°58′38.10“W	NE Atlantic- English Channel	19	6	3	0.468 ± 0.140	0.00204 ± 0.0007	0.1667*
Ferrol†	FER	8–10	43°27′25.27“N 8°20′9.07“W	NE Atlantic	16	7	4	0.775 ± 0.088	0.00391 ± 0.0008	0.1650**
Vigo-Cornide†	COR	30–60	42°13′3.81“N 8°49′41.24“W	NE Atlantic	22	7	4	0.481 ± 0.131	0.00206 ± 0.0007	0.1618**
Atlantic basin					79	21	16	0.625 ± 0.063	0.00294 ± 0.0004	0.1040*
Blanes†	ABL	90	41°40′2.19“N 2°52′30.44“E	NW Mediterranean	9	4	2	0.750 ± 0.139	0.00821 ± 0.0033	0.2036
Indemares†	IMA	123–139	41° 18,5752′ N 2°25,6324′ E	NW Mediterranean	5	2	1	0.400 ± 0.237	0.00388 ± 0.0023	0.3000
Planassa†	PLA	80–100	41°38′47.92“N 2°54′40.61“E	NW Mediterranean	21	12	8	0.824 ± 0.084	0.01235 ± 0.0022	0.1345
Port de la Selva†	PSE	80–100	42°21′59.49“N 3°13′26.37“E	NW Mediterranean	23	4	1	0.577 ± 0.090	0.00849 ± 0.0012	0.1341**
Mediterranean basin					58	17	12	0.698 ± 0.065	0.00960 ± 0.0012	0.1037*
TOTAL					137	34	28	0.777 ± 0.029	0.00979 ± 0.0005	0.0088*
Ophiothrixsp. II
Ferrol†	FER	8–10	43°27′25.27“N 8°20′9.07“W	NE Atlantic	33	12	7	0.758 ± 0.122	0.00408 ± 0.0011	0.1112*
Cascais	CAS	Intertidal	38°42′25.77“N 9°29′15.41“W	NE Atlantic	18	11	5	0.810 ± 0.093	0.00409 ± 0.0008	0.0539**
Armação de Pera	APA	10–15	37°5′41.52“N 8°21′37.31“W	NE Atlantic	20	9	3	0.758 ± 0.101	0.00394 ± 0.0009	0.0541**
Ceuta	CEB	18–25	35°52′52.19“N 5°19′10.20“W	Gibraltar Strait	19	8	3	0.672 ± 0.119	0.00321 ± 0.0009	0.0857**
La Herradura	LH	15–18	36°22′4.36“N 5°12′51.68“W	SW Mediterranean	20	6	2	0.447 ± 0.137	0.00241 ± 0.0009	0.0921*
Xábea	XB	10–20	38°47′39.48“N 0°12′18.10“E	SW Mediterranean	20	7	3	0.521 ± 0.135	0.00219 ± 0.0007	0.0818**
Roses	RS	10–20	42°14′11.17“N 3°15′45.80“E	NW Mediterranean	14	4	1	0.494 ± 0.151	0.00172 ± 0.0006	0.1246*
Cadaqués	CAD	2–15	42°17′27.85“N 3°17′43.95“E	NW Mediterranean	19	9	5	0.772 ± 0.094	0.00407 ± 0.0009	0.0700**
Alcudia	AL	15–18	39°52′1.28“N 3°11′39.87“E	W Mediterranean-Balearic Is.	26	13	5	0.720 ± 0.099	0.00359 ± 0.0008	0.0412**
Ladiko	LK	2–10	36°19′22.22“N 28°13′18.81“E	E Mediterranean	16	9	5	0.817 ± 0.095	0.00459 ± 0.0013	0.0745**
Kalytea Bay	KB	2–5	35°9′58.03“N 24°24′38.20“E	E Mediterranean	9	6	3	0.833 ± 0.126	0.00418 ± 0.0012	0.1111**
TOTAL					215	58	42	0.679 ± 0.0390	0.00345 ± 0.00031	0.0117**
Ophiothrixsp. III
Demersales-45†	DEM45	153	43°36′44.7“N 8°29′49.5“W	NE Atlantic- Cantabrian Sea	20	6	3	0.679 ± 0.101	0.0040 ± 0.0009	0.098*
Demersales-91†	DEM91	310	43°53′27.24“N 5°34′53.77“W	NE Atlantic- Cantabrian Sea	22	5	5	0.745 ± 0.093	0.0039 ± 0.0007	0.1486**
Demersales-131†	DEM131	131	43°30′57.0“N 2°53′32.4“W	NE Atlantic- Cantabrian Sea	23	8	5	0.744 ± 0.080	0.0039 ± 0.0006	0.1472**
Peniche†	POR	100–150	39°27′53“N 9°12′48.33“W	NE Atlantic	7	4	1	0.714 ± 0.181	0.0054 ± 0.0018	0.2417
PINDALBV17†	PINDAL	169	36°08′0“N 30°2′0”E	SW Mediterranean	8	4	1	0.750 ± 0.139	0.0036 ± 0.0008	0.2311**
TOTAL					80	19	15	0.714 ± 0.047	0.00407 ± 0.0004	0.100**

N number of individuals, H number of haplotypes, p private haplotypes, Hd haplotype diversity, π nucleotide diversity, R² Ramos-Onsins & Rozas statistic.

For Ophiothrix sp. II and Ophiothrix sp. III information of the Atlantic and Mediterranean basins is not presented since these two species did not displayed significant differences between geographical areas. ^†New samples sequenced in this study. ^*p < 0.05 and ^**p < 0.01.