FIG. 4.
Transposable elements in response to ambient PM exposure. A and C, DNA methylation in LINE-1 (L1) and SINE B1/B2 transposable elements was measured by methylation-sensitive qPCR assay. B and D, The differential expression of LINE-1 and SINE B1/B2 in murine macrophages was determined by qRT-PCR. The fold change data were calculated from the ΔΔCt values. All qRT-PCR reactions were conducted in triplicate and repeated twice. Asterisks (*) denotes significant (P < .05), (**) denotes significant (P < .01), and (***) denotes significant (P < .001) difference from control.