FIG. 5.
Satellite DNA is affected by exposure to ambient PM. A and D, The differential expression of major and minor satellites in murine macrophages was determined by qRT-PCR. B and E, DNA methylation major and minor satellites was measured by methylation-sensitive qRT-PCR assay. C and F, The differential expression of Uhrf1 and Suv39h1 was determined by qRT-PCR. The fold change data were calculated from the ΔΔCt values. All qRT-PCR reactions were conducted in triplicate and repeated twice. Asterisks (*) denotes significant (P < .05), (**) denotes significant (P < .01), and (***) denotes significant (P < .001) difference from control.