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. 2015 Apr 21;21(7):553–562. doi: 10.1093/molehr/gav022

Figure 1.

Figure 1

Confirmation of the absence of the complete AZFc region in testis tissues of all AZFc-deleted patients. The complete absence or presence of the AZFc region in testicular genomic DNA of all patients and controls was performed in a multiplex PCR by testing six STS markers. Primer set 1 contains sY142, sY1191 and sY1197. Primer set 2 contains sY1206, sY1291 and sY1201. Absence of sY1191, sY1206 and sY1291 in the presence of sY142, sY1197 and sY1201 (LANES 4–9) confirmed the AZFc deletion in our azoospermic patients. All controls had no deletion (LANES 1–3).