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. 2015 Apr 21;21(7):553–562. doi: 10.1093/molehr/gav022

Figure 3.

Figure 3

Relative gene expression of germ cell markers in non-deleted (controls) and AZFc-deleted short-term and long-term cultured and MACS sorted HLA− germ cells. (A) Spermatogonial marker expression: ubiquitin carboxyl-terminal esterase L1 (UCHL1), zinc finger and BTB domain containing 16 (ZBTB16), GDNF family receptor alpha 1 (GFRA1) and melanoma antigen family A4 (MAGEA4). (B) Germ cell differentiation marker expression: signal transducer and activator of transcription 3 (STAT3), spermatogenesis and oogenesis specific basic helix-loop-helix 2 (SOHLH2), v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT) and synaptonemal complex protein 3 (SYCP3). Polymerase (RNA) II polypeptide A (POLR2A) was used as the reference gene. Data expressed as mean ± SEM compared with the short-term or long-term cultured control cells. Asterisks highlight significant differences with P< 0.05.