FIG. 3.

Localization of miR-21, -200c, and 423 in human kidney biopsies from patients with ATN. Pathological examination of the biopsy samples was performed with standard H&E stained sections. Using in situ hybridization, the expression patterns of miR-21, -200c, and -423 were assessed in kidney biopsies from normal controls and patients diagnosed with ATN. In situ hybridization settings were proven with U6 as positive control, having an abundant nuclear expression, and a scrabbled probe, not complementary to any known miRNA sequence, used as negative control. Star, tubular casts; arrow, positive miR-21 signal. For in situ: blue, positive probe staining; pink, counter staining; H&E pictures taken with 10× others with 20x objective; representative images of n = 3 controls and n = 3 ATN patients.